| Name | Latitude | Longitude | GNIS Map Designation |
| Ames Church of the Nazarene | 420117N | 0933651W | Ames East |
| Ames Community Church | 420213N | 0933639W | Ames East |
| Ames Four Square Church | 420242N | 0933721W | Ames East |
| Ames Four Square Church | 420130N | 0933651W | Ames East |
| Ames Mennonite Church | 420205N | 0933712W | Ames East |
| Ames Reformed Church | 420239N | 0933751W | Ames West |
| Ames Seventh Day Adventist Church | 420205N | 0933714W | Ames East |
| Ascension Lutheran Church | 420140N | 0933651W | Ames East |
| Assembly of God Church | 420128N | 0932657W | Nevada |
| Bergen Lutheran Church | 421001N | 0933009W | Story City |
| Bethany Lutheran Church | 415937N | 0933610W | Huxley |
| Bethany Lutheran Church | 420954N | 0932334W | McCallsburg |
| Bethany Lutheran Church | 415702N | 0933955W | Slater |
| Bethel Lutheran Church | 421119N | 0933538W | Story City |
| Bethel United Methodist Church | 420841N | 0931741W | Zearing |
| Bethesda Lutheran Church | 420215N | 0933735W | Ames West |
| Bethlehem Lutheran Church | 415252N | 0934059W | Slater |
| Bible Faith Church | 421100N | 0933553W | Story City |
| Brethren Church | 415326N | 0932400W | Maxwell |
| Bridgeway Church | 420255N | 0933841W | Ames West |
| Calvary United Methodist Church | 420247N | 0933746W | Ames West |
| Campus Baptist Church | 420205N | 0933712W | Ames East |
| Central Presbyterian Church | 420114N | 0932714W | Nevada |
| Chinese Evangelical Free Church | 420239N | 0933813W | Ames West |
| Christian Church | 415320N | 0932355W | Maxwell |
| Christian Church | 420055N | 0932739W | Nevada |
| Collegiate Presbyterian Church | 420131N | 0933918W | Ames West |
| Collegiate United Methodist Church | 420118N | 0933913W | Ames West |
| Collins Christian Church | 415408N | 0931819W | Collins |
| Community Evangelical Free Church | 420150N | 0932650W | Nevada |
| Community of Christ Church | 420123N | 0933941W | Ames West |
| Cornerstone Church of Ames | 420009N | 0933625W | Ames East |
| Cornerstone Church of Christ | 420944N | 0931741W | Zearing |
| Evangelical Free Church | 420125N | 0933858W | Ames West |
| Faith Baptist Church | 415354N | 0933152W | Huxley |
| First Assembly of God Church | 420206N | 0933701W | Ames East |
| First Baptist Church of Ames | 420115N | 0933845W | Ames West |
| First Christian Church | 420138N | 0933703W | Ames East |
| First Church of Christ Scientist | 420158N | 0933649W | Ames East |
| First Evangelical Free Church | 420239N | 0933813W | Ames West |
| First Presbyterian Church | 415322N | 0932355W | Maxwell |
| First United Methodist Church | 420135N | 0933649W | Ames East |
| Fjeldberg Lutheran Church | 415338N | 0933619W | Huxley |
| Friendship Baptist Church | 420042N | 0933909W | Ames West |
| Gilbert Evangelical Free Church | 420625N | 0933903W | Ames West |
| Gilbert Lutheran Church | 420625N | 0933826W | Ames West |
| Grace Evangelical Free Church | 415343N | 0933603W | Huxley |
| Grace United Methodist Church | 421117N | 0933537W | Story City |
| Grand Avenue Baptist Church | 420243N | 0933711W | Ames East |
| Harvest Assembly of God Church | 415347N | 0933605W | Huxley |
| Heartland Baptist Church | 420205N | 0933712W | Ames East |
| Immanuel Lutheran Church | 421115N | 0933531W | Story City |
| Korean Christian Reformed Church | 420159N | 0934005W | Ames West |
| Korean United Methodist Church | 420205N | 0933712W | Ames East |
| Lord of Life Lutheran Church | 420150N | 0933633W | Ames East |
| McCallsburg Presbyterian Church | 420954N | 0932314W | McCallsburg |
| Memorial Lutheran Church | 420120N | 0933846W | Ames West |
| Memorial Lutheran Church | 420102N | 0932638W | Nevada |
| Nazareth Lutheran Church | 415406N | 0933203W | Huxley |
| Nevada Baptist Church | 420117N | 0932657W | Nevada |
| New Life United Pentecostal Church | 420205N | 0933712W | Ames East |
| North Grand Church of Christ | 420306N | 0933726W | Ames East |
| Northminster Presbyterian Church | 420230N | 0933749W | Ames West |
| Oakwood Road Church | 415950N | 0933910W | Slater |
| Palestine Church | 415242N | 0933454W | Huxley |
| Pleasant Grove Church | 420624N | 0933341W | Ames East |
| Reorganized Church of Jesus Christ of Latter Day Saints | 420123N | 0933941W | Ames West |
| Saint Andrews Lutheran Church | 420128N | 0933949W | Ames West |
| Saint Cecilia Church | 420259N | 0933746W | Ames West |
| Saint Gabriel's Catholic Church | 420926N | 0931751W | Zearing |
| Saint Johns by the Campus Church | 420120N | 0933853W | Ames West |
| Saint Patrick's Catholic Church | 420121N | 0932648W | Nevada |
| Saint Paul Lutheran Church | 420212N | 0933709W | Ames East |
| Saint Petri Lutheran Church | 421124N | 0933526W | Story City |
| Saint Thomas Aquinas Catholic Church | 420121N | 0933843W | Ames West |
| Saints Peter and Paul Church | 420853N | 0934154W | Ames NW |
| Salem Lutheran Church | 420959N | 0933000W | McCallsburg |
| Seventh Day Adventist Church | 420036N | 0932707W | Nevada |
| Sheldahl Norwegian Lutheran Church | 415159N | 0934144W | Polk City |
| Slater Baptist Church | 415240N | 0934043W | Slater |
| Stonbrook Community Church | 420324N | 0933817W | Ames West |
| Story City Evangelical Free Church | 420959N | 0933007W | Story City |
| The Church of Jesus Christ of Latter Day Saints | 420250N | 0933746W | Ames West |
| Trinity Christian Reformed Church | 420159N | 0934005W | Ames West |
| Trinity United Methodist Church | 415358N | 0933623W | Huxley |
| United Church of Christ | 420138N | 0933649W | Ames East |
| United Methodist Church | 415246N | 0934101W | Slater |
| United Methodist Church | 415331N | 0932359W | Maxwell |
| United Methodist Church | 415410N | 0931823W | Collins |
| United Methodist Church | 415353N | 0933159W | Huxley |
| United Methodist Church | 420117N | 0932705W | Nevada |
| Unity Church of Ames | 420132N | 0933646W | Ames East |
| University Lutheran Congregational Church | 420116N | 0933835W | Ames West |
| Westside Church of Church | 420115N | 0934031W | Ames West |
| Willow Creek Open Bible Church | 420117N | 0934036W | Ames West |
| Zearing Christian Church | 420941N | 0931757W | Zearing |
